Translational_Unit
LytC-Heli-
Part:BBa_K316044:Design
Designed by: IC 2010 Team Group: iGEM10_Imperial_College_London (2010-10-27)
Pveg-spoVG-LytC-Helical Linker-TEV Cleavage Site-AIP-His Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
LytC cell wall binding domain BBa_K316030 was extracted from ‘’Bacillus subtilis’’ genome using PCR with Pfu DNA polymerase. The linker, TEV protease cleavage site and his-tagged auto inducing peptide were produced by direct synthesis by mwg Eurofins[http://www.eurofinsdna.com/home.html]. The two parts were combined using AccI[http://www.neb.com/nebecomm/products/productR0161.asp] restriction endonuclease.